Negativibacillus massiliensis gen. nov., sp. nov., a New Bacterial Genus Isolated from a Human Left Colon Sample

Camille Valles (1) , Morgane Mailhe (2) , Davide Ricaboni (1) , Nicholas Armstrong (1) , Stéphane Alibar (2) , Véronique Vitton , Jean-Christophe Lagier (1) , Didier Raoult (1) , Maryam Tidjani Alou (1)

1 MEPHI - Microbes évolution phylogénie et infections
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection

A new genus, a member of the Ruminococcaceae family, was isolated from the left colon of a healthy woman. Strain Marseille P3213 was a non-motile, spore-forming, Gram-stain negative, rod-shaped bacterium. This strictly anaerobic species reached optimal growth after an incubation of 72 h at 37 °C. The 16S rRNA gene sequence of this strain shared a 93.52% similarity level with Harryflintia acetispora strain V20-281a, its closest phylogenetic neighbor with standing in the nomenclature. Its genome had a size of 2.87 Mb, with a 45.81% G + C content. We hereby propose the creation of Negativibacillus massiliensis strain P3213T as the 43rd genus within the Ruminococcaceae family.

Keywords: Negativibacillus massiliensis; new genus; culturomics; taxono-genomics; genome

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology; Life Sciences [q-bio]; Microbiology and Parasitology; Virology; Life Sciences [q-bio]; Microbiology and Parasitology; Parasitology; Life Sciences [q-bio]; Human health and pathology; Cardiology and cardiovascular system; Life Sciences [q-bio]; Human health and pathology; Emerging diseases; Life Sciences [q-bio]; Human health and pathology; Infectious diseases

Camille Valles, Morgane Mailhe, Davide Ricaboni, Nicholas Armstrong, Stéphane Alibar, et al.. Negativibacillus massiliensis gen. nov., sp. nov., a New Bacterial Genus Isolated from a Human Left Colon Sample. Microbiology Research, 2021, 12 (1), pp.29-42. ⟨10.3390/microbiolres12010004⟩. ⟨hal-03279632⟩