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Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report

Abstract : Current routine real-time PCR methods used for the point-of-care diagnosis of infectious meningitis do not allow for one-shot genotyping of the pathogen, as in the case of deadly Haemophilus influenzae meningitis. Real-time PCR diagnosed H. influenzae meningitis in a 22-year-old male patient, during his hospitalisation following a more than six-metre fall. Using an Oxford Nanopore Technologies real-time sequencing run in parallel to real-time PCR, we detected the H. influenzae genome directly from the cerebrospinal fluid sample in six hours. Furthermore, BLAST analysis of the sequence encoding for a partial DUF417 domain-containing protein diagnosed a non-b serotype, non-typeable H.influenzae belonging to lineage H. influenzae 22.1-21. The Oxford Nanopore metagenomic next-generation sequencing approach could be considered for the point-of-care diagnosis of infectious meningitis, by direct identification of pathogenic genomes and their genotypes/serotypes.
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https://hal-amu.archives-ouvertes.fr/hal-03280490
Contributor : Isabelle Combe <>
Submitted on : Wednesday, July 7, 2021 - 1:30:36 PM
Last modification on : Thursday, July 8, 2021 - 3:30:01 AM

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Madjid Morsli, Quentin Kerharo, Jeremy Delerce, Pierre-Hugues Roche, Lucas Troude, et al.. Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report. Pathogens, MDPI, 2021, 10 (4), pp.461. ⟨10.3390/pathogens10040461⟩. ⟨hal-03280490⟩

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