Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Pathogens Année : 2021

Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report

Madjid Morsli
  • Fonction : Auteur
Quentin Kerharo
  • Fonction : Auteur
Pierre-Hugues Roche
  • Fonction : Auteur
Lucas Troude

Résumé

Current routine real-time PCR methods used for the point-of-care diagnosis of infectious meningitis do not allow for one-shot genotyping of the pathogen, as in the case of deadly Haemophilus influenzae meningitis. Real-time PCR diagnosed H. influenzae meningitis in a 22-year-old male patient, during his hospitalisation following a more than six-metre fall. Using an Oxford Nanopore Technologies real-time sequencing run in parallel to real-time PCR, we detected the H. influenzae genome directly from the cerebrospinal fluid sample in six hours. Furthermore, BLAST analysis of the sequence encoding for a partial DUF417 domain-containing protein diagnosed a non-b serotype, non-typeable H.influenzae belonging to lineage H. influenzae 22.1-21. The Oxford Nanopore metagenomic next-generation sequencing approach could be considered for the point-of-care diagnosis of infectious meningitis, by direct identification of pathogenic genomes and their genotypes/serotypes.
Fichier principal
Vignette du fichier
pathogens-10-00461.pdf (546.2 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-03280490 , version 1 (22-06-2023)

Licence

Paternité

Identifiants

Citer

Madjid Morsli, Quentin Kerharo, Jeremy Delerce, Pierre-Hugues Roche, Lucas Troude, et al.. Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report. Pathogens, 2021, 10 (4), pp.461. ⟨10.3390/pathogens10040461⟩. ⟨hal-03280490⟩
21 Consultations
12 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More