Direct Diagnosis of Echovirus 12 Meningitis Using Metagenomic Next Generation Sequencing

Madjid Morsli (1, 2) , Christine Zandotti (2) , Aurelie Morand (3) , Philippe Colson (4, 2) , Michel Drancourt (1, 2)

1 MEPHI - Microbes évolution phylogénie et infections
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
3 Service de pédiatrie spécialisée et médecine infantile (neurologie, pneumologie, maladies héréditaires du métabolisme) [Hôpital de la Timone - APHM]
4 VITROME - Vecteurs - Infections tropicales et méditerranéennes

The current point-of-care diagnosis of enterovirus meningitis does not identify the viral genotype, which is prognostic. In this case report, more than 81% of an Echovirus 12 genome were detected and identified by metagenomic next-generation sequencing, directly from the cerebrospinal fluid collected in a 6-month-old child with meningeal syndrome and meningitis: introducing Echovirus 12 as an etiological agent of acute meningitis in the pediatric population.

Mots clés: enterovirus meningitis; whole genome sequencing; metagenomic next-generation sequencing; Echovirus 12; cerebrospinal fluid

Domaines: Bactériologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses; Parasitologie; Virologie

Madjid Morsli, Christine Zandotti, Aurelie Morand, Philippe Colson, Michel Drancourt. Direct Diagnosis of Echovirus 12 Meningitis Using Metagenomic Next Generation Sequencing. Pathogens, 2021, 10 (5), pp.610. ⟨10.3390/pathogens10050610⟩. ⟨hal-03280504⟩