Skip to Main content Skip to Navigation
Journal articles

Scanning Electron Microscope: A New Potential Tool to Replace Gram Staining for Microbe Identification in Blood Cultures

Abstract : Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard. We developed a new rapid strategy using a tabletop scanning electron microscope (SEM) and compared its performance with Gram staining for the detection of micro-organisms and preliminary identification directly from blood cultures. We first optimised the sample preparation for twelve samples simultaneously, saving time on imaging. In this work, SEM proved its ability to identify bacteria and yeasts in morphotypes up to the genus level in some cases. We blindly tested 1075 blood cultures and compared our results to the Gram staining preliminary identification, with MALDI-TOF/MS as a reference. This method presents major advantages such as a fast microbe identification, within an hour of the blood culture being detected positive, low preparation costs, and data traceability. This SEM identification strategy can be developed into an automated assay from the sample preparation, micrograph acquisition, and identification process. This strategy could revolutionise urgent microbiological diagnosis of infectious diseases.
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03333970
Contributor : Isabelle Combe Connect in order to contact the contributor
Submitted on : Friday, September 3, 2021 - 12:40:49 PM
Last modification on : Tuesday, October 19, 2021 - 10:50:36 PM

Links full text

Identifiers

Collections

Citation

Gabriel Haddad, Sara Bellali, Tatsuki Takakura, Anthony Fontanini, Yusuke Ominami, et al.. Scanning Electron Microscope: A New Potential Tool to Replace Gram Staining for Microbe Identification in Blood Cultures. Microorganisms, MDPI, 2021, 9 (6), pp.1170. ⟨10.3390/microorganisms9061170⟩. ⟨hal-03333970⟩

Share

Metrics

Record views

45