SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades

Ahmad Ibrahim (1) , Lucie Peyclit (1) , Rim Abdallah (1) , Saber Khelaifia (1) , Amanda Chamieh (1) , Jean-Marc Rolain (1) , Fadi Bittar (1)

1 MEPHI - Microbes évolution phylogénie et infections

Candida auris is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium for all C. auris clades. This medium is sensitive with a limit of detection ranging between 101 and 102 CFU/mL. The 100% specificity of SCA (specific C. auris) medium is confirmed on a set of 135 Candida strains, 50 bacterial species and 200 human stool samples. Thus, this medium specifically selects for C. auris isolation from clinical samples, allowing the latter to study its phenotypic profile.

Keywords: Candida; Candida auris; culture; emerging fungus; isolation; specific medium

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology; Life Sciences [q-bio]; Microbiology and Parasitology; Virology; Life Sciences [q-bio]; Microbiology and Parasitology; Parasitology; Life Sciences [q-bio]; Human health and pathology; Cardiology and cardiovascular system; Life Sciences [q-bio]; Human health and pathology; Emerging diseases; Life Sciences [q-bio]; Human health and pathology; Infectious diseases

Ahmad Ibrahim, Lucie Peyclit, Rim Abdallah, Saber Khelaifia, Amanda Chamieh, et al.. SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades. Journal of Fungi, 2021, 7 (6), pp.433. ⟨10.3390/jof7060433⟩. ⟨hal-03333972⟩