BODY-ON-A-CHIP: ON-CHIP HEART RECEIVING METABOLITES FROM ON-CHIP LIVER

Adam Williamson (1) , Uta Fernekorn (1) , Sukhdeep Singh (1) , Andreas Schober (1)

1 TU - Technische Universität Ilmenau

We report on the development of an integrated microfluidic and multi-electrode array (MEA) system housed in a microbioreactor for Body-on-a-Chip applications. On a single chip, we successfully demonstrate the use of this system by connecting an on-Chip Liver and an on-Chip Heart. We demonstrate that acylated dihydropyrimidinone (DHPM), when added to the on-Chip Liver, is deacylated and results in DHPM, a well-known calcium channel blocker, delivered to the on-Chip Heart by on-Chip organ-to-organ interconnectivity. Deacylated DHPM delivery to the cardiomyocytes is verified by MEA measurements of cardiomyocyte electroactivity and Liquid chromatography-mass spectrometry (LCMS) of the cardiomyocyte medium.

Keywords: P19 Stem Cells; Cardiomyocytes; Hepatocytes; Organ-on-a-Chip

Domains: Life Sciences [q-bio]

Adam Williamson, Uta Fernekorn, Sukhdeep Singh, Andreas Schober. BODY-ON-A-CHIP: ON-CHIP HEART RECEIVING METABOLITES FROM ON-CHIP LIVER. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Oct 2013, Freiburg, Germany. ⟨hal-03349096⟩