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Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study

Pénélope Bourgoin 1, 2 Inès Ait Belkacem 3, 2 Isabelle Arnoux 4 Pierre-Emmanuel Morange 1, 4 Fabrice Malergue 2
1 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
2 Beckman Coulter Immunotech, Marseille, France
3 CIML - Centre d'Immunologie de Marseille - Luminy
4 TIMONE - Hôpital de la Timone [CHU - APHM]
Abstract : Aim: A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Methods: Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. Results: All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. Conclusion: The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.
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https://hal-amu.archives-ouvertes.fr/hal-03374568
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Submitted on : Tuesday, October 12, 2021 - 11:04:46 AM
Last modification on : Tuesday, October 19, 2021 - 10:59:27 PM

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Pénélope Bourgoin, Inès Ait Belkacem, Isabelle Arnoux, Pierre-Emmanuel Morange, Fabrice Malergue. Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study. Future Microbiology, Future Medicine, 2021, 16 (13), pp.955-966. ⟨10.2217/fmb-2021-0034⟩. ⟨hal-03374568⟩

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