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Journal articles

GATA1 pathogenic variants disrupt MYH10 silencing during megakaryopoiesis

Abstract : Background GATA1 is an essential transcription factor for both polyploidization and megakaryocyte (MK) differentiation. The polyploidization defect observed in GATA1 variant carriers is not well understood. Objective To extensively phenotype two pedigrees displaying different variants in the GATA1 gene and determine if GATA1 controls MYH10 expression levels, a key modulator of MK polyploidization. Method A total of 146 unrelated propositi with constitutional thrombocytopenia were screened on a multigene panel. We described the genotype-phenotype correlation in GATA1 variant carriers and investigated the effect of these novel variants on MYH10 transcription using luciferase constructs. Results The clinical profile associated with the p.L268M variant localized in the C terminal zinc finger was unusual in that the patient displayed bleeding and severe platelet aggregation defects without early-onset thrombocytopenia. p.N206I localized in the N terminal zinc finger was associated, on the other hand, with severe thrombocytopenia (15G/L) in early life. High MYH10 levels were evidenced in platelets of GATA1 variant carriers. Analysis of MKs anti-GATA1 chromatin immunoprecipitation-sequencing data revealed two GATA1 binding sites, located in the 3′ untranslated region and in intron 8 of the MYH10 gene. Luciferase reporter assays showed their respective role in the regulation of MYH10 gene expression. Both GATA1 variants significantly alter intron 8 driven MYH10 transcription. Conclusion The discovery of an association between MYH10 and GATA1 is a novel one. Overall, this study suggests that impaired MYH10 silencing via an intronic regulatory element is the most likely cause of GATA1-related polyploidization defect.
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https://hal-amu.archives-ouvertes.fr/hal-03374701
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Submitted on : Friday, October 22, 2021 - 10:13:19 AM
Last modification on : Friday, April 1, 2022 - 3:57:14 AM
Long-term archiving on: : Sunday, January 23, 2022 - 6:51:15 PM

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Paul Saultier, Sandrine Cabantous, Michel Puceat, Franck Peiretti, Timothée Bigot, et al.. GATA1 pathogenic variants disrupt MYH10 silencing during megakaryopoiesis. Journal of Thrombosis and Haemostasis, Wiley, 2021, 19 (9), pp.2287-2301. ⟨10.1111/jth.15412⟩. ⟨hal-03374701⟩

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