Rab7b regulates dendritic cell migration by linking lysosomes to the actomyosin cytoskeleton - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2021

Rab7b regulates dendritic cell migration by linking lysosomes to the actomyosin cytoskeleton

Résumé

Lysosomal signaling facilitates the migration of immune cells by releasing Ca 2+ to activate the actin-based motor myosin II at the cell rear. However, how the actomyosin cytoskeleton physically associates to lysosomes is unknown. We have previously identified myosin II as a direct interactor of Rab7b, a small GTPase that mediates the transport from late endosomes/lysosomes to the trans-Golgi network (TGN). Here, we show that Rab7b regulates the migration of dendritic cells (DCs) in one-and three-dimensional environments. DCs are immune sentinels that transport antigens from peripheral tissues to lymph nodes to activate T lymphocytes and initiate adaptive immune responses. We found that the lack of Rab7b reduces myosin II light chain phosphorylation and the activation of the transcription factor EB (TFEB), which controls lysosomal signaling and is required for fast DC migration. Furthermore, we demonstrate that Rab7b interacts with the lysosomal Ca 2+ channel TRPML1 (also known as MCOLN1), enabling the local activation of myosin II at the cell rear. Taken together, our findings identify Rab7b as the missing physical link between lysosomes and the actomyosin cytoskeleton, allowing control of immune cell migration through lysosomal signaling. This article has an associated First Person interview with the first author of the paper.
Fichier principal
Vignette du fichier
jcs259221.pdf (8.05 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-03389465 , version 1 (21-10-2021)

Licence

Paternité

Identifiants

Citer

Katharina Vestre, Irene Persiconi, Marita Borg Distefano, Nadia Mensali, Noemi Antonella Guadagno, et al.. Rab7b regulates dendritic cell migration by linking lysosomes to the actomyosin cytoskeleton. Journal of Cell Science, 2021, 134 (18), pp.jcs259221. ⟨10.1242/jcs.259221⟩. ⟨hal-03389465⟩
75 Consultations
27 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More