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Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity $in\ vitro$

Abstract : Long-terminal repeat (LTR) retrotransposons are genetic elements that, like retroviruses, replicate by reverse transcription of an RNA intermediate into a complementary DNA (cDNA) that is next integrated into the host genome by their own integrase. The Ty1 LTR retrotransposon has proven to be a reliable working model to investigate retroelement integration site preference. However, the low yield of recombinant Ty1 integrase production reported so far has been a major obstacle for structural studies. Here we analyze the biophysical and biochemical properties of a stable and functional recombinant Ty1 integrase highly expressed in $E.coli$. The recombinant protein is monomeric and has an elongated shape harboring the three-domain structure common to all retroviral integrases at the N-terminal half, an extra folded region, and a large intrinsically disordered region at the C-terminal half. Recombinant Ty1 integrase efficiently catalyzes concerted integration $in\ vitro$, and the N-terminal domain displays similar activity. These studies that will facilitate structural analyses may allow elucidating the molecular mechanisms governing Ty1 specific integration into safe places in the genome.
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https://hal-amu.archives-ouvertes.fr/hal-03402697
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Submitted on : Tuesday, October 26, 2021 - 10:05:52 AM
Last modification on : Monday, November 28, 2022 - 11:08:08 AM

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Phong Quoc Nguyen, Christine Conesa, Elise Rabut, Gabriel Bragagnolo, Célia Gouzerh, et al.. Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity $in\ vitro$. Journal of Biological Chemistry, 2021, 297 (4), pp.101093. ⟨10.1016/j.jbc.2021.101093⟩. ⟨hal-03402697⟩

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