Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles

Lu Ren (1) , Hongtao Ji (2) , Karine Heuzé (2) , Bruno Faure (1) , Emilie Genin (2) , Pierre Rousselot Pailley (1) , Thierry Tron (1)

1 ISM2 - Institut des Sciences Moléculaires de Marseille
2 ISM - Institut des Sciences Moléculaires

We explored the coupling of laccases to magnetic nanoparticles (MNPs) with different surface chemical coating. Two laccase variants offering two opposite and precise orientations of the substrate oxidation site were immobilised onto core-shell MNPs presenting either aliphatic aldehyde, aromatic aldehyde or azide functional groups at the particles surface. Oxidation capabilities of the six-resulting laccase-MNP hybrids were compared on ABTS and coniferyl alcohol. Herein, we show that the original interfaces created differ substantially in their reactivities with an amplitude from 1 to > 4 folds depending on the nature of the substrate. Taking enzyme orientation into account in the design of surface modification represents a way to introduce selectivity in laccase catalysed reactions.

Mots clés: Magnetic nano-particles; Chemical coating; Laccase orientation; Oxidations

Domaines: Catalyse; Chimie inorganique; Biochimie [q-bio.BM]

Lu Ren, Hongtao Ji, Karine Heuzé, Bruno Faure, Emilie Genin, et al.. Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles. Colloids and Surfaces B: Biointerfaces, 2021, 206, pp.111963. ⟨10.1016/j.colsurfb.2021.111963⟩. ⟨hal-03405996⟩