HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents

Abstract : Organophosphorus nerve agents (OPNAs) are highly toxic compounds inhibiting cholinergic enzymes in the central and autonomic nervous systems and neuromuscular junctions, causing severe intoxications in humans. Medical countermeasures and efficient decontamination solutions are needed to counteract the toxicity of a wide spectrum of harmful OPNAs including G, V and Novichok agents. Here, we describe the use of engineered OPNA-degrading enzymes for the degradation of various toxic agents including insecticides, a series of OPNA surrogates, as well as real chemical warfare agents (cyclosarin, sarin, soman, tabun, VX, A230, A232, A234). We demonstrate that only two enzymes can degrade most of these molecules at high concentrations (25 mM) in less than 5 min. Using surface assays adapted from NATO AEP-65 guidelines, we further show that enzyme-based solutions can decontaminate 97.6% and 99.4% of 10 g∙m−2 of soman- and VX-contaminated surfaces, respectively. Finally, we demonstrate that these enzymes can degrade ethyl-paraoxon down to sub-inhibitory concentrations of acetylcholinesterase, confirming their efficacy from high to micromolar doses.
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03428685
Contributor : Isabelle Combe Connect in order to contact the contributor
Submitted on : Monday, November 15, 2021 - 11:30:58 AM
Last modification on : Friday, April 1, 2022 - 3:44:10 AM

Links full text

Identifiers

Collections

Citation

Pauline Jacquet, Benjamin Rémy, Rowdy Bross, Marco van Grol, Floriane Gaucher, et al.. Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents. International Journal of Molecular Sciences, MDPI, 2021, 22 (15), pp.8152. ⟨10.3390/ijms22158152⟩. ⟨hal-03428685⟩

Share

Metrics

Record views

8