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Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

Abstract : This paper presents the development and the application of a multisyringe flow injection analysis system for the fluorimetric determination of the major heat-stable known allergen in shrimp, rPen a 1 (tropomyosin). This muscle protein, made up of 284 amino acids, is the main allergen in crustaceans and can be hydrolyzed by microwave in hydrochloric acid medium to produce glutamic acid, the major amino acid in the protein. Glutamic acid can then be quantified specifically by thermal conversion into pyroglutamic acid followed by chemical derivatization of the pyroglutamic acid formed by an analytical protocol based on an OPA-NAC reagent. Pyroglutamic acid can thus be quantified between 1 and 100 M in less than 15 min with a detection limit of 1.3 µM. The method has been validated by measurements on real samples demonstrating that the response increases with the increase in the tropomyosin content or with the increase in the mass of the shrimp sample.
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https://hal-amu.archives-ouvertes.fr/hal-03478106
Contributor : Jean-Luc Boudenne Connect in order to contact the contributor
Submitted on : Monday, December 13, 2021 - 6:10:07 PM
Last modification on : Sunday, June 26, 2022 - 3:24:56 AM
Long-term archiving on: : Monday, March 14, 2022 - 7:37:07 PM

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Bruno Coulomb, Fabien Robert-Peillard, Najib Ben Ali Gam, Salwa Sadok, Jean-Luc Boudenne. Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples. Molecules, MDPI, 2021, 26, ⟨10.3390/molecules26195809⟩. ⟨hal-03478106⟩

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