Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid

Jacek Zielonka (1) , Adam Sikora (2) , Radoslaw Podsiadly (2) , Micael Hardy (3) , B. Kalyanaraman (1)

1 MCW - Medical College of Wisconsin [Milwaukee]
2 Łódź University of Technology
3 ICR - Institut de Chimie Radicalaire

The development of boronic probes enabled reliable detection and quantitative analysis of hydrogen peroxide , other nucleophilic hydroperoxides, hypochlorite , and peroxynitrite . The major product, in which boronate moiety of the probe is replaced by the hydroxyl group, is, however, common for all those oxidants. Here, we describe how ortho-isomer of mitochondria-targeted phenylboronic acid can be used to detect and differentiate peroxynitrite-dependent and independent probe oxidation. This method highlights detection and quantification of both the major, phenolic product and the minor, peroxynitrite-specific cyclic and nitrated products of probe oxidation.

Domaines: Chimie

Jacek Zielonka, Adam Sikora, Radoslaw Podsiadly, Micael Hardy, B. Kalyanaraman. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. Volkmar Weissig; Marvin Edeas. Mitochondrial Medicine, Volume 1: Targeting Mitochondria, 2275 (20), Springer; Humana, pp.315-327, 2021, Methods in Molecular Biology, 978-1-0716-1261-3. ⟨10.1007/978-1-0716-1262-0_20⟩. ⟨hal-03627767⟩