Culturomics revealed the bacterial constituents of the microbiota of a 10-year-old laboratory culture of planarian species S. mediterranea

Luis Johnson Kangale (1, 2) , Didier Raoult (1, 3) , Pierre-Edouard Fournier (1, 2) , Eric Ghigo (1)

1 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
2 VITROME - Vecteurs - Infections tropicales et méditerranéennes
3 MEPHI - Microbes évolution phylogénie et infections

The planarian species Schmidtea mediterranea is a flatworm living in freshwater that is used in the research laboratory as a model to study developmental and regeneration mechanisms, as well as antibacterial mechanisms. However, the cultivable microbial repertoire of the microbes comprising its microbiota remains unknown. Here, we characterized the bacterial constituents of a 10-year-old laboratory culture of planarian species S. mediterranea via culturomics analysis. We isolated 40 cultivable bacterial species, including 1 unidentifiable species. The predominant phylum is Proteobacteria , and the most common genus is Pseudomonas . We discovered that parts of the bacterial flora of the planarian S. mediterranea can be classified as fish pathogens and opportunistic human pathogens.

Mots clés: Microbial communities; Microbial ecology; Microbiome

Domaines: Bactériologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses; Parasitologie; Virologie

Luis Johnson Kangale, Didier Raoult, Pierre-Edouard Fournier, Eric Ghigo. Culturomics revealed the bacterial constituents of the microbiota of a 10-year-old laboratory culture of planarian species S. mediterranea. Scientific Reports, 2021, 11 (1), pp.24311. ⟨10.1038/s41598-021-03719-5⟩. ⟨hal-03636885⟩