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Article Dans Une Revue Nature Communications Année : 2021

Application of 3D MAPs pipeline identifies the morphological sequence chondrocytes undergo and the regulatory role of GDF5 in this process

Sarah Rubin
Johannes Stegmaier
  • Fonction : Auteur
Neta Felsenthal
Jonathan Svorai
  • Fonction : Auteur
Yoseph Addadi
Tomer Stern
Elazar Zelzer

Résumé

Abstract The activity of epiphyseal growth plates, which drives long bone elongation, depends on extensive changes in chondrocyte size and shape during differentiation. Here, we develop a pipeline called 3D Morphometric Analysis for Phenotypic significance (3D MAPs), which combines light-sheet microscopy, segmentation algorithms and 3D morphometric analysis to characterize morphogenetic cellular behaviors while maintaining the spatial context of the growth plate. Using 3D MAPs, we create a 3D image database of hundreds of thousands of chondrocytes. Analysis reveals broad repertoire of morphological changes, growth strategies and cell organizations during differentiation. Moreover, identifying a reduction in Smad 1/5/9 activity together with multiple abnormalities in cell growth, shape and organization provides an explanation for the shortening of Gdf5 KO tibias. Overall, our findings provide insight into the morphological sequence that chondrocytes undergo during differentiation and highlight the ability of 3D MAPs to uncover cellular mechanisms that may regulate this process.

Dates et versions

hal-03638208 , version 1 (12-04-2022)

Identifiants

Citer

Sarah Rubin, Ankit Agrawal, Johannes Stegmaier, Sharon Krief, Neta Felsenthal, et al.. Application of 3D MAPs pipeline identifies the morphological sequence chondrocytes undergo and the regulatory role of GDF5 in this process. Nature Communications, 2021, 12 (1), pp.5363. ⟨10.1038/s41467-021-25714-0⟩. ⟨hal-03638208⟩
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