Journal articles
A Unique Bacterial Secretion Machinery with Multiple Secretion Centers
Abstract : The Porphyromonas gingivalis type IX secretion system (T9SS) promotes periodontal disease by secreting gingipains and other virulence factors. By in situ cryoelectron tomography, we report that the P. gingivalisT9SS consists of eighteen PorM dimers arranged as a large caged ring in the periplasm. Near the outer membrane, PorM dimers interact with a PorKN ring complex of ~52 nm in diameter. PorMKN translocation complexes of a given T9SS adopt distinct conformations energized by the proton motive force, suggestive of different activation states. At the inner membrane, PorM associates with a cytoplasmic complex that exhibits 12-fold symmetry and requires both PorM and PorL for assembly. Activated motors deliver substrates across the outer membrane via one of eight Sov translocons arranged in a ring. The T9SSs are unique among known secretion systems in bacteria and eukaryotes in their assembly as supramolecular machines composed of apparently independently-functioning translocation motors and export pores.
https://hal-amu.archives-ouvertes.fr/hal-03655004
Contributor : Eric Cascales Connect in order to contact the contributor
Submitted on : Friday, April 29, 2022 - 10:31:50 AM
Last modification on : Saturday, April 30, 2022 - 3:35:47 AM
Contributor : Eric Cascales Connect in order to contact the contributor
Submitted on : Friday, April 29, 2022 - 10:31:50 AM
Last modification on : Saturday, April 30, 2022 - 3:35:47 AM
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