Evaluation of the Rotating-Frame Relaxation ( T 1ρ ) Filter and Its Application in Metabolomics as an Alternative to the Transverse Relaxation ( T 2 ) Filter

Elena Piersanti (1) , Lamya Rezig (1) , Fabrice Tranchida (1) , Wael El-Houri (1) , Seidou Abagana (1) , Mylène Campredon (1) , Laetitia Shintu (1) , Mehdi Yemloul (1)

1 ISM2 - Institut des Sciences Moléculaires de Marseille

NMR-based metabolomic studies commonly involve the use of T2-filter pulse sequences to eliminate or attenuate the broad signals from large molecules and improve spectral resolution. In this paper, we demonstrate that T1ρ filter-based pulse sequence represents an interesting alternative since it allows the stability and the reproducibility needed for statistical analysis. The integrity of the samples and the stability of the instruments were assessed for different filter durations and amplitudes. We showed that the T1ρ filter pulse sequence did not induce sample overheating for a filter duration up to 500 ms. The reproducibility was evaluated and compared with T2-filter in serum and liver samples. The implementation is relatively simple and provides the same statistical and analytical results as those obtained with the standard filters. Regarding tissues analysis, since the duration of the filter is that of the spin-lock, the synchronization of the echo delays with the Magic Angle Spinning (MAS) rate is no longer necessary as for T2 based filter sequences. The results presented in this article aim at establishing a new protocol to improve metabolomic studies and pave the way for future developments on T1ρ alternative filters, on liquid and HR-MAS NMR experiments.

Domaines: Chimie

Elena Piersanti, Lamya Rezig, Fabrice Tranchida, Wael El-Houri, Seidou Abagana, et al.. Evaluation of the Rotating-Frame Relaxation ( T 1ρ ) Filter and Its Application in Metabolomics as an Alternative to the Transverse Relaxation ( T 2 ) Filter. Analytical Chemistry, 2021, 93 (25), pp.8746-8753. ⟨10.1021/acs.analchem.0c05251⟩. ⟨hal-03655575⟩