MalaSelect: A Selective Culture Medium for Malassezia Species - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Journal of Fungi Année : 2021

MalaSelect: A Selective Culture Medium for Malassezia Species

Résumé

Malassezia species are fastidious and slow-growing yeasts in which isolation from polymicrobial samples is hampered by fast-growing microorganisms. Malassezia selective culture media are needed. Although cycloheximide is often used, some fungi, including the chief human commensal Candida albicans, are resistant to this compound. This study aimed to test whether the macrolide rapamycin could be used in combination with cycloheximide to develop a Malassezia-selective culture medium. Rapamycin susceptibility testing was performed via microdilution assays in modified Dixon against two M. furfur and five Candida spp. The MIC was the lowest concentration that reduced growth by a minimum of 90%. Rapamycin ± cycloheximide 500 mg/L was also added to FastFung solid, and yeast suspensions were inoculated and incubated for 72 h. Rapamycin MICs for Candida spp. ranged from 0.5 to 2 mg/L, except for C. krusei, for which the MIC was >32 mg/L. M. furfur stains were rapamycin-resistant. Rapamycin and cycloheximide supplementation of the FastFung medium effectively inhibited the growth of non-Malassezia yeast, including cycloheximide-resistant C. albicans and C. tropicalis. Based on our findings, this “MalaSelect” medium should be further evaluated on polymicrobial samples for Malassezia isolation and culture.
Fichier principal
Vignette du fichier
jof-07-00824.pdf (1.87 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-03662927 , version 1 (28-06-2023)

Licence

Paternité

Identifiants

Citer

Abdourahim Abdillah, Stéphane Ranque. MalaSelect: A Selective Culture Medium for Malassezia Species. Journal of Fungi, 2021, 7 (10), pp.824. ⟨10.3390/jof7100824⟩. ⟨hal-03662927⟩
5 Consultations
24 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More