Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings

Hanane Zerrouki (1, 2, 3) , Sid-Ahmed Rebiahi (1) , Yamina Elhabiri (1) , Ahlam Fatmi (4) , Sophie Alexandra Baron (2, 3) , Isabelle Pagnier (2, 3) , Seydina Diene (2, 3) , Jean-Marc Rolain (2, 3)

1 Université Aboubekr Belkaid - University of Belkaïd Abou Bekr [Tlemcen]
2 MEPHI - Microbes évolution phylogénie et infections
3 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
4 CHU de Tlemcen - Centre hospitalier universitaire de Tlemcen (Algérie)

(1) Background: The purpose of this study was to determine the prevalence of clostridia strains in a hospital environment in Algeria and to evaluate their antimicrobial susceptibility to antibiotics and biocides. (2) Methods: Five hundred surface samples were collected from surfaces in the intensive care unit and surgical wards in the University Hospital of Tlemcen, Algeria. Bacterial identification was carried out using MALDI-TOF-MS, and then the minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by the E-test method. P. sordellii toxins were searched by enzymatic and PCR assays. Seven products intended for daily disinfection in the hospitals were tested against Clostridium spp. spore collections. (3) Results: Among 100 isolates, 90 P. sordellii were identified, and all strains were devoid of lethal and hemorrhagic toxin genes. Beta-lactam, linezolid, vancomycin, tigecycline, rifampicin, and chloramphenicol all proved effective against isolated strains. Among all strains tested, the spores of P. sordellii exhibited remarkable resistance to the tested biocides compared to other Clostridium species. The (chlorine-based 0.6%, 30 min), (glutaraldehyde solution 2.5%, 30 min), and (hydrogen peroxide/peracetic acid 3%, 15 min) products achieved the required reduction in spores. (4) Conclusions: Our hospital’s current cleaning and disinfection methods need to be optimized to effectively remove spores from caregivers’ hands, equipment, and surfaces.

Mots clés: Paeniclostridium sordellii; hospital environment; MICs; antibiotics; disinfectant products; antimicrobial resistance

Domaines: Bactériologie; Cardiologie et système cardiovasculaire; Maladies émergentes; Maladies infectieuses; Parasitologie; Virologie

Hanane Zerrouki, Sid-Ahmed Rebiahi, Yamina Elhabiri, Ahlam Fatmi, Sophie Alexandra Baron, et al.. Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings. Antibiotics, 2022, 11 (1), pp.38. ⟨10.3390/antibiotics11010038⟩. ⟨hal-03663710⟩