Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays - Archive ouverte HAL Access content directly
Journal Articles STAR Protocols Year : 2021

Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

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Abstract

Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation.
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hal-03663914 , version 1 (10-05-2022)

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Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Clémence Grosjean, Julie Quessada, Mathis Nozais, Marie Loosveld, Dominique Payet-Bornet, et al.. Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays. STAR Protocols, 2021, 2 (4), pp.100961. ⟨10.1016/j.xpro.2021.100961⟩. ⟨hal-03663914⟩
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