Skip to Main content Skip to Navigation
New interface
Journal articles

Population Diversity of Antibiotic Resistant Enterobacterales in Samples From Wildlife Origin in Senegal: Identification of a Multidrug Resistance Transposon Carrying blaCTX–M–15 in Escherichia coli

Abstract : Introduction The role of wildlife in the transmission of antimicrobial resistant (AMR) is suspected but scarcely reported in current studies. Therefore, we studied the dynamics and prevalence of antibiotic-resistant Enterobacterales in antibiotic-limited areas of Senegal. Materials and Methods We collected fecal samples from monkeys and apes (N = 226) and non-fecal environmental samples (N = 113) from Senegal in 2015 and 2019. We grew the samples on selective media, subsequently isolated AMR Enterobacterales , and then sequenced their genomes. Results We isolated 72 different Enterobacterales among which we obtained a resistance rate of 65% for colistin (N = 47/72) and 29% for third generation-cephalosporin (C3G) (29%, N = 21/72). Interestingly, almost 46% of our isolates, among Enterobacter sp., Citrobacter cronae and Klebsiella aerogenes , belong to 34 new STs. Moreover, the genes bla CTX – M –15 , bla TEM 1 B , sul2 , dfrA14 , qnrs , aph ( 3′′ ), aph ( 6 ), tetA , and tetR harbored within a transposon on the IncY plasmid of ST224 Escherichia coli were transferred and inserted into a ST10 E . coli phage coding region. Conclusion Wildlife constitutes a rich, unexplored reservoir of natural microbial diversity, AMR genes and international resistant clones pathogenic in humans. The presence of a transposon that carries AMR genes is intriguing since no antibiotics are used in the non-human primates we studied.
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03678929
Contributor : Isabelle COMBE Connect in order to contact the contributor
Submitted on : Wednesday, May 25, 2022 - 4:23:25 PM
Last modification on : Tuesday, August 23, 2022 - 11:48:10 AM

Links full text

Identifiers

Collections

Citation

Rim Abdallah, Edmond Kuete Yimagou, Linda Hadjadj, Oleg Mediannikov, Ahmad Ibrahim, et al.. Population Diversity of Antibiotic Resistant Enterobacterales in Samples From Wildlife Origin in Senegal: Identification of a Multidrug Resistance Transposon Carrying blaCTX–M–15 in Escherichia coli. Frontiers in Microbiology, 2022, 13, ⟨10.3389/fmicb.2022.838392⟩. ⟨hal-03678929⟩

Share

Metrics

Record views

14