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Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus

Abstract : The successive emergence of highly pathogenic coronaviruses (CoVs) such as the Severe Acute Respiratory Syndrome (SARS-CoV) in 2003 and the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012 has stimulated a number of studies on the molecular biology. This research has provided significant new insight into functions and activities of the replication/transcription multi-protein complex. The latter directs both continuous and discontinuous RNA synthesis to replicate and transcribe the large coronavirus genome made of a single-stranded, positive-sense RNA of similar to 30 kb. In this review, we summarize our current understanding of SARS-CoV enzymes involved in RNA biochemistry, such as the in vitro characterization of a highly active and processive RNA polymerase complex which can associate with methyltransferase and 3'-5' exoribonuclease activities involved in RNA capping, and RNA proofreading, respectively. The recent discoveries reveal fascinating RNA-synthesizing machinery, highlighting the unique position of coronaviruses in the RNA virus world.
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https://hal-amu.archives-ouvertes.fr/hal-03794372
Contributor : Isabelle Imbert Connect in order to contact the contributor
Submitted on : Monday, October 3, 2022 - 11:17:22 AM
Last modification on : Wednesday, November 30, 2022 - 3:57:55 AM

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Marion Sevajol, Lorenzo Subissi, Etienne Decroly, Bruno Canard, Isabelle Imbert. Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus. Virus Research, 2014, 194, pp.90-99. ⟨10.1016/j.virusres.2014.10.008⟩. ⟨hal-03794372⟩

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