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Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2014

One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

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1
Lorenzo Subissi
  • Function : Author
Clara Posthuma
  • Function : Author
Axelle Collet
  • Function : Author
Jessika Zevenhoven-Dobbe
  • Function : Author
Alexander Gorbalenya
  • Function : Author
Etienne Decroly
  • Function : Author
Eric Snijder
  • Function : Author
Bruno Canard
  • Function : Author

Abstract

Significance The 2003 severe acute respiratory syndrome (SARS) epidemic and recent emergence of Middle East respiratory syndrome highlight the potential lethality of zoonotic coronavirus infections in humans. No specific antiviral treatment options are available. Coronaviruses possess the largest known RNA virus genomes and encode a complex replication machinery consisting of 16 viral nonstructural proteins (nsps). Our study reveals that the SARS-coronavirus RNA polymerase (nsp12) needs to associate with nsp7 and nsp8 to activate its capability to replicate long RNA. Moreover, this complex associates with nsp14, the proofreading subunit required to safeguard coronavirus replication fidelity. Our study thus defines the core of an RNA-synthesizing machinery that is unique in the RNA virus world and includes several key targets for antiviral drug development.

Dates and versions

hal-03794373 , version 1 (03-10-2022)

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Lorenzo Subissi, Clara Posthuma, Axelle Collet, Jessika Zevenhoven-Dobbe, Alexander Gorbalenya, et al.. One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (37), ⟨10.1073/pnas.1323705111⟩. ⟨hal-03794373⟩
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