Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from Litsea glutinosa: In Vitro and In Vivo Studies

Shafia Shafiq (1) , Ronok Zahan (1) , Samina Yesmin , Alam Khan , Md. Sabbir Mahmud , Md Abu Reza , Sarah Albogami , Mohammed Alorabi , Michel de Waard , Hebatallah Saad , Jean-Marc Sabatier (2) , Tarannum Naz , Gaber El-Saber Batiha

1 University of Rajshahi
2 INP - Institut de neurophysiopathologie

Litsea glutinosa (L. glutinosa) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, L. glutinosa, were examined using non-toxic doses and a phytochemical analysis was executed using gas-chromatography–mass-spectrometry. The in vitro antioxidant study of the L. glutinosa methanolic extract (LGBME) revealed a concentration-dependent antioxidant property. The bark extract showed promising antioxidant effects in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest antioxidant activity was demonstrated at the maximum concentration (50 µg/mL). The IC50 values of the LGBME and BHT were 5.51 and 5.01 µg/mL, respectively. At the same concentration, the total antioxidant capacity of the LGBME was 0.161 µg/mL and the ferric reducing antioxidant power assay result of the LGBME was 1.783 µg/mL. In the cytotoxicity study, the LD50 of the LGBME and gallic acid were 24.93 µg/mL and 7.23 µg/mL, respectively. In the in vivo anticancer-activity studies, the LGBME, particularly at a dose of 150 mg/kg/bw, showed significant cell-growth inhibition, decreased tumor weight, increased mean survival rate, and upregulated the reduced hematological parameters in EAC (Ehrlich’s ascites carcinoma)-induced Swiss albino mice. The highest cell-growth inhibition, 85.76%, was observed with the dose of 150 mg/kg/bw. Furthermore, the upregulation of pro-apoptotic genes (p53, Bax) and the downregulation of anti-apoptotic Bcl-2 were observed. In conclusion, LGBME extract has several bioactive phytoconstituents, which confirms the antioxidant and anticancer properties of L. glutinosa.

Mots clés: antioxidant; DPPH; anticancer; apoptosis; gas-chromatography–mass-spectrometry; ethnomedicinal plants

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Cancer

Shafia Shafiq, Ronok Zahan, Samina Yesmin, Alam Khan, Md. Sabbir Mahmud, et al.. Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from Litsea glutinosa: In Vitro and In Vivo Studies. Molecules, 2022, 27 (20), pp.6964. ⟨10.3390/molecules27206964⟩. ⟨hal-03823671⟩