Skip to Main content Skip to Navigation
New interface
Journal articles

BTN3A Targeting Vγ9Vδ2 T Cells Antimicrobial Activity Against Coxiella burnetii-Infected Cells

Abstract : Vγ9Vδ2 T cells have been reported to participate to the immune response against infectious diseases such as the Q fever caused by Coxiella burnetii infection. Indeed, the number and proportion of Vγ9Vδ2 T cells are increased during the acute phase of Q fever. Human Vγ9Vδ2 T cell responses are triggered by phosphoantigens (pAgs) produced by pathogens and malignant cells, that are sensed via the membrane receptors butyrophilin-3A1 (BTN3A1) and -2A1 (BTN2A1). Here, by using CRISPR-Cas9 inactivation in THP-1 cells, we show that BTN3A and BTN2A are required to Vγ9Vδ2 T cell response to C. burnetii infection, though not directly involved in the infection process. Furthermore, C. burnetii -infected monocytes display increased BTN3A and BTN2A expression and induce Vγ9Vδ2 T cell activation that can be inhibited by specific antagonist mAb. More importantly, we show that the antimicrobial functions of Vγ9Vδ2 T cells towards C. burnetii are enhanced in the presence of an BTN3A activating antibody. This supports the role of Vγ9Vδ2 T cells in the control of C. burnetii infection and argues in favor of targeting these cells as an alternative treatment strategy for infectious diseases caused by intracellular bacteria.
Complete list of metadata

https://hal-amu.archives-ouvertes.fr/hal-03823828
Contributor : Isabelle COMBE Connect in order to contact the contributor
Submitted on : Friday, October 21, 2022 - 11:13:17 AM
Last modification on : Tuesday, October 25, 2022 - 3:44:43 AM

Links full text

Identifiers

Collections

Citation

Laetitia Gay, Soraya Mezouar, Carla Cano, Etienne Foucher, Mélanie Gabriac, et al.. BTN3A Targeting Vγ9Vδ2 T Cells Antimicrobial Activity Against Coxiella burnetii-Infected Cells. Frontiers in Immunology, 2022, 13, ⟨10.3389/fimmu.2022.915244⟩. ⟨hal-03823828⟩

Share

Metrics

Record views

0