Weizmannia faecalis sp. nov., isolated from a human stool sample

Hoang Thong Kieu (1, 2) , Thi Phuong Thao Pham , Cheikh Ibrahima Lo (1, 2) , Stéphane Alibar (2) , Ludivine Bréchard (2) , Nicholas Armstrong (1, 2) , Philippe Decloquement (1) , Aldiouma Diallo (3) , Cheikh Sokhna (2, 3) , Matthieu Million (1, 2) , Jean-Christophe Lagier (2, 1) , Didier Raoult (1, 2) , Maryam Tidjani Alou

1 MEPHI - Microbes évolution phylogénie et infections
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
3 VITROME - Vecteurs - Infections tropicales et méditerranéennes

Using the culturomics approach, the previously unknown strain Marseille-P8953T, was isolated and classified within the Weizmannia genus. Strain Marseille-P8953T was isolated from the faeces of a healthy subject and consisted of Gram-stain positive, spore-forming, motile rod-shaped cells. A 99.2% similarity was observed between the 16S rRNA gene of strain Marseille-P8953T (accession number LR735539) and that of Weizmannia coagulans strain NBRC 12583T (accession number KX261624), its closest phylogenetic relative, while the genome of strain Marseille-P8953T (3.5 Mpb long, 46.5% GC content) shared the average nucleotide identity by Orthology and digital DNA-DNA Hybridisation values of 95 and 60.4%, respectively. Given the phylogenetic classification and phenotypic characteristics of strain Marseille-P8953T, we propose the creation of a new species within the Weizmannia genus named Weizmannia faecalis (= CSUR P8953T = CECT 9904 T).

Mots clés: Culturomics; Gut microbiota; New species; Taxono-genomics; Weizmannia faecalis sp. nov.

Domaines: Sciences du Vivant [q-bio]

Hoang Thong Kieu, Thi Phuong Thao Pham, Cheikh Ibrahima Lo, Stéphane Alibar, Ludivine Bréchard, et al.. Weizmannia faecalis sp. nov., isolated from a human stool sample. Archives of Microbiology, 2022, 204 (10), pp.612. ⟨10.1007/s00203-022-03229-6⟩. ⟨hal-03941949⟩