Prevotella merdae sp. nov., a new bacterial species isolated from human faeces

Mossaab Maaloum (1) , Pamela Afouda (2) , Cheikh Ibrahima Lo (2, 3) , Gregory Dubourg (2, 3) , Thi Tien Nguyen (3) , Anthony Levasseur (2, 3) , Rachid Saile (4) , Didier Raoult (2, 3) , Pierre-Edouard Fournier (3, 1)

1 VITROME - Vecteurs - Infections tropicales et méditerranéennes
2 MEPHI - Microbes évolution phylogénie et infections
3 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
4 University Hassan II [Casablanca]

Abstract Strain Marseille-P4119T was isolated from a faecal sample of a healthy 32-year-old faecal transplant donor. The bacterium was anaerobic, Gram-negative, rod-shaped, non-motile, and did not produce spores. We studied its phenotypic characteristics and sequenced its whole genome. The major fatty acids were C15:0anteiso and C15:0iso. The final genome assembly was 3912650 bp long with a 44.4 mol% G + C content, 3094 protein-coding genes and 74 RNA genes. Strain Marseille-P4119T exhibited a 97.10% 16S rRNA sequence identity and a 29.0% dDDH with Prevotella stercorea CB35T, OrthoANI values ranged from 68.5% with Prevotella enoeca to 77.4% with Prevotella stercorea, the phylogenetically closest bacterial species with standing in nomenclature. Based on the phylogenetic, phenotypic and genomic analyses, we propose the creation of the novel species Prevotella merdae sp. nov. The type strain is Marseille-P4119T ( = CSUR P4119T = CECT 9566T).

Domaines: Sciences du Vivant [q-bio]

Mossaab Maaloum, Pamela Afouda, Cheikh Ibrahima Lo, Gregory Dubourg, Thi Tien Nguyen, et al.. Prevotella merdae sp. nov., a new bacterial species isolated from human faeces. FEMS Microbiology Letters, 2022, 369 (1), pp.fnac066. ⟨10.1093/femsle/fnac066⟩. ⟨hal-03948695⟩