CRISPR-Based Detection, Identification and Typing of Mycobacterium tuberculosis Complex Lineages

A. Padane (1, 2) , Z. Harouna Hamidou (2, 3) , M. Drancourt (2, 4) , J. Saad (2, 4)

1 IRESSEF - Institut de Recherche en Santé, de Surveillance Épidémiologique et de Formation [Dakar, Sénégal]
2 MEPHI - Microbes évolution phylogénie et infections
3 Laboratoire National de Référence des IST/VIH et de la Tuberculose
4 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection

The whole-genome sequence comparison of the Mycobacterium tuberculosis complex (MTC) genomic sequences that are available in the NCBI database identified a unique, specific gene to be used directly on clinical diagnostic samples to detect MTC against all species of mycobacteria and to differentiate between MTC species, lineages, and sublineages.

Domains: Life Sciences [q-bio]

A. Padane, Z. Harouna Hamidou, M. Drancourt, J. Saad. CRISPR-Based Detection, Identification and Typing of Mycobacterium tuberculosis Complex Lineages. Microbiology Spectrum, 2023, Jan 5, pp.e0271722. ⟨10.1128/spectrum.02717-22⟩. ⟨hal-03985939⟩