Combined ESR and Thermodynamic Studies of the Superoxide Adduct of 5-(Diethoxyphosphoryl)-5-Methyl-1-Pyrroline N-Oxide (DEPMPO): Hindered Rotation around the O-O Bond Evidenced by Two-Dimensional Simulation of Temperature-Dependent Spectra

Antal Rockenbauer (1) , Jean-Louis Clément (2) , Marcel Culcasi (2) , Anne Mercier (2) , Paul Tordo (2) , Sylvia Pietri (2)

1 Chemical Research Center, Institute of Chemistry, Budapest
2 ICR - Institut de Chimie Radicalaire

Experiments were performed to elucidate the origin of the superhyperfine structure and line width alternation (LWA) seen in the ESR spectrum of the major diastereoisomer (1) of DEPMPO-OOH, the remarkably persistent superoxide adduct of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO). Using selectively deuterated DEPMPO derivatives, we demonstrated that the superhyperfine pattern can be unambiguously attributed to long-range couplings. The recording in pyridine of highly resolved spectra in a wide temperature range, combined with two-dimensional simulation, allowed us to characterize an inverted LWA in 1 and revealed a uniform line broadening in the spectrum of the minor DEPMPO-OOH diastereoisomer (2), with both effects originating from a chemical exchange between conformers. When the individual spectra of 1 presenting LWA in the fast-exchange regime were simulated, four equally good fits were obtained and this ambiguity could be resolved by using a two-dimensional simulation technique. The thermodynamic and kinetic constants of this exchange were consistent with a rotation around the O-O bond. We propose that line broadening effects in 1 and 2 result from this O-O rotation concerted with the pseudo-rotation of the pyrrolidine ring.

Domaines: Sciences du Vivant [q-bio]; Chimie théorique et/ou physique

Antal Rockenbauer, Jean-Louis Clément, Marcel Culcasi, Anne Mercier, Paul Tordo, et al.. Combined ESR and Thermodynamic Studies of the Superoxide Adduct of 5-(Diethoxyphosphoryl)-5-Methyl-1-Pyrroline N-Oxide (DEPMPO): Hindered Rotation around the O-O Bond Evidenced by Two-Dimensional Simulation of Temperature-Dependent Spectra. Journal of Physical Chemistry A, 2007, 111, pp.4950. ⟨10.1021/jp070679u⟩. ⟨hal-01142288⟩