Skip to Main content Skip to Navigation
Journal articles

The Functional Organization of Neocortical Networks Investigated in Slices with Local Field Recordings and Laser Scanning Photostimulation

Abstract : The organization of cortical networks can be investigated functionally in brain slices. Laser scanning photostimulation (LSPS) with glutamate-uncaging allows for a rapid survey of all connections impinging on single cells recorded in patch-clamp. We sought to develop a variant of the method that would allow for a more exhaustive mapping of neuronal networks at every experiment. We found that the extracellular field recordings could be used to detect synaptic responses evoked by LSPS. One to two electrodes were placed in all six cortical layers of barrel cortex successively and maps were computed from the size of synaptic negative local field potentials. The field maps displayed a laminar organization similar to the one observed in maps computed from excitatory postsynaptic currents recorded in patch-clamp mode. Thus, LSPS combined with field recording is an interesting alternative to obtain for every animal tested a comprehensive map of the excitatory intracortical network.
Complete list of metadatas

Cited literature [41 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01201713
Contributor : Administrateur Hal Amu <>
Submitted on : Thursday, September 17, 2015 - 5:50:18 PM
Last modification on : Tuesday, February 4, 2020 - 10:00:04 AM
Long-term archiving on: : Tuesday, December 29, 2015 - 8:33:36 AM

File

fetchObject.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Melissa A. Erlandson, Olivier J. Manzoni, Ingrid Bureau. The Functional Organization of Neocortical Networks Investigated in Slices with Local Field Recordings and Laser Scanning Photostimulation. PLoS ONE, Public Library of Science, 2015, pp.1-17. ⟨10.1371/journal.pone.0132008.g005⟩. ⟨hal-01201713⟩

Share

Metrics

Record views

156

Files downloads

340