Innate and Adaptive Immune Functions of Peyer's Patch Monocyte-Derived Cells Article Innate and Adaptive Immune Functions of Peyer's Patch Monocyte-Derived Cells

Johnny Bonnardel (1) , Clément da Silva (1) , Sandrine Henri (1) , Samira Tamoutounour (1) , Lionel Chasson (1) , Frederic Montañana-Sanchis (1) , Jean-Pierre Gorvel (1) , Hugues Lelouard (1)

1 CIML - Centre d'Immunologie de Marseille - Luminy

Peyer's patches (PPs) are primary inductive sites of mucosal immunity. Defining PP mononuclear phagocyte system (MPS) is thus crucial to understand the initiation of mucosal immune response. We provide a comprehensive analysis of the phenotype, distribution, ontogeny, lifespan, function, and transcriptional profile of PP MPS. We show that monocytes give rise to macrophages and to lysozyme-expressing dendritic cells (LysoDCs), which are both involved in particulate antigen uptake, display strong innate antiviral and antibacterial gene signatures, and, upon TLR7 stimulation, secrete IL-6 and TNF, but neither IL-10 nor IFN gamma. However, unlike macrophages, LysoDCs display a rapid renewal rate, strongly express genes of the MHCII presentation pathway, and prime naive helper T cells for IFNg production. Our results show that monocytes differentiate locally into LysoDCs and macrophages, which display distinct features from their adjacent villus counterparts.

Keywords: DENDRITIC CELLS; T-CELLS; BACTERIAL-INFECTION; TISSUE MACROPHAGES; GENE-EXPRESSION; BONE-MARROW; IN-VIVO; B-CELLS; RECEPTOR; ANTIGEN

Domains: Life Sciences [q-bio]; Cellular Biology

Johnny Bonnardel, Clément da Silva, Sandrine Henri, Samira Tamoutounour, Lionel Chasson, et al.. Innate and Adaptive Immune Functions of Peyer's Patch Monocyte-Derived Cells Article Innate and Adaptive Immune Functions of Peyer's Patch Monocyte-Derived Cells. Cell Reports, 2015, pp.770-784. ⟨10.1016/j.celrep.2015.03.067⟩. ⟨hal-01212685⟩