Journal articles
The Repertoire of Archaea Cultivated from Severe Periodontitis
Abstract : In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.
Cited literature [21 references]
https://hal-amu.archives-ouvertes.fr/hal-01216665
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, October 16, 2015 - 4:05:41 PM
Last modification on : Thursday, March 4, 2021 - 3:08:38 AM
Long-term archiving on: : Monday, January 18, 2016 - 6:05:30 AM
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, October 16, 2015 - 4:05:41 PM
Last modification on : Thursday, March 4, 2021 - 3:08:38 AM
Long-term archiving on: : Monday, January 18, 2016 - 6:05:30 AM
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