The Repertoire of Archaea Cultivated from Severe Periodontitis

Hong T. T. Huynh (1, 2) , Marion Pignoly (2) , Vanessa D. Nkamga (1) , Michel Drancourt (1) , Gérard Aboudharam (1, 2)

1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2 AMU ODONTO - Aix-Marseille Université - Faculté d'odontologie

In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.

Keywords: 16S RIBOSOMAL-RNA; METHANOGENIC ARCHAEA; METHANOBREVIBACTER-ORALIS; QUANTITATIVE-ANALYSIS; DENTAL PLAQUE; GENE ANALYSIS; MCRA GENE; SP-NOV; DIVERSITY; IDENTIFICATION

Domains: Life Sciences [q-bio]; Microbiology and Parasitology

Hong T. T. Huynh, Marion Pignoly, Vanessa D. Nkamga, Michel Drancourt, Gérard Aboudharam. The Repertoire of Archaea Cultivated from Severe Periodontitis. PLoS ONE, 2015, 10 (e0121565), ⟨10.1371/journal.pone.0121565.t001⟩. ⟨hal-01216665⟩