Skip to Main content Skip to Navigation
Journal articles

Gorilla gorilla gorilla gut: a potential reservoir of pathogenic bacteria as revealed using culturomics and molecular tools

Abstract : Wild apes are considered to be the most serious reservoir and source of zoonoses. However, little data are available about the gut microbiota and pathogenic bacteria in gorillas. For this propose, a total of 48 fecal samples obtained from 21 Gorilla gorilla gorilla individuals (as revealed via microsatellite analysis) were screened for human bacterial pathogens using culturomics and molecular techniques. By applying culturomics to one index gorilla and using specific media supplemented by plants, we tested 12,800 colonies and identified 147 different bacterial species, including 5 new species. Many opportunistic pathogens were isolated, including 8 frequently associated with human diseases; Mycobacterium bolletii, Proteus mirabilis, Acinetobacter baumannii, Klebsiella pneumoniae, Serratia marcescens, Escherichia coli, Staphylococcus aureus and Clostridium botulinum. The genus Treponema accounted for 27.4% of the total reads identified at the genus level via 454 pyrosequencing. Using specific real-time PCR on 48 gorilla fecal samples, in addition to classical human pathogens, we also observed the fastidious bacteria Bartonella spp. Borrelia spp., Coxiella burnetii and Tropheryma whipplei in the gorilla population. We estimated that the prevalence of these pathogens vary between 4.76% and 85.7%. Therefore, gorillas share many bacterial pathogens with humans suggesting that they could be a reservoir for their emergence.
Document type :
Journal articles
Complete list of metadatas

Cited literature [39 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01241992
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, December 11, 2015 - 11:44:56 AM
Last modification on : Wednesday, May 29, 2019 - 1:40:02 PM
Long-term archiving on: : Saturday, March 12, 2016 - 1:00:15 PM

File

srep07174.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Fadi Bittar, Mamadou B. Keita, Jean-Christophe Lagier, Martine Peeters, Eric Delaporte, et al.. Gorilla gorilla gorilla gut: a potential reservoir of pathogenic bacteria as revealed using culturomics and molecular tools. Scientific Reports, Nature Publishing Group, 2014, 4 (7174 ), ⟨10.1038/srep07174⟩. ⟨hal-01241992⟩

Share

Metrics

Record views

254

Files downloads

389