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Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions

Elisa Roca 1, 2, 3 Romaric Lacroix 2, 3 Coralie Judicone Sophie Laroumagne Stéphane Robert 2, 3 Sylvie Cointe 2, 3 Alexandre Muller 2, 3 Elise Kaspi Patrice Roll Alain R Brisson 4 Claudio Tantucci Philippe Astoul Francoise Dignat-George 5, 2, 3
1 Università degli Studi di Brescia [Brescia]
2 Physiopathologie de l'Endothelium
VRCM - Vascular research center of Marseille
3 VRCM - Vascular research center of Marseille
4 CBMN - Chimie et Biologie des Membranes et des Nanoobjets
5 LA CONCEPTION - Hôpital de la Conception [CHU - APHM]
Abstract : Pleural biomarkers allowing to mini-invasively discriminate benign from malignant pleural effusions are needed. Among potential candidates, microparticles (MPs) are extracellular vesicles that vectorize antigen derived from the parent cell. We hypothesized that tumor-derived MPs could be present in the pleural liquid and help to identify patients with malignant pleural effusions. Using highly sensitive flow cytometry and cryo-electron microscopy, we showed that large amounts of MPs from hematopoïetic and vascular origin could be detectable in pleural fluids. Their level did not differ between benign (n = 14) and malignant (n = 71) pleural effusions. Analysis of selected tumoral associated antigens (podoplanin, mucin 1 and EpCAM, epithelial-cell-adhesion-molecule) evidenced for the first time the presence of tumor-derived MPs expressing EpCAM in malignant pleural fluids only (Specificity = 93%, Sensitivity = 49% and 45% for flow cytometry and ELISA, respectively). The detection of EpCAM-positive-MPs (EpCAM + MPs) by flow cytometry showed a better specificity and sensitivity than ELISA to distinguish between pleural carcinoma and the others malignant pleural effusions (MPE; Sp: 96% vs 89%; Se: 79% vs 66%). Combining EpCAM+ MPs and cytology improved the diagnosis of MPE compared to cytology alone. This study establishes the basis for using EpCAM+ MPs as a promising new biomarker that could be added to the armamentarium to mini-invasively identify patients with malignant pleural effusions.
Keywords : microparticles pleural effusion pleural neoplasia EpCAM extracellular vesicles
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Journal articles
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https://hal-amu.archives-ouvertes.fr/hal-01455550
Contributor : Françoise Dignat-George Connect in order to contact the contributor
Submitted on : Friday, February 3, 2017 - 4:07:27 PM
Last modification on : Monday, December 20, 2021 - 4:44:01 PM

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  • HAL Id : hal-01455550, version 1
  • PUBMED : 26689993

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UNIV-AMU | CNRS | INC-CNRS

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Elisa Roca, Romaric Lacroix, Coralie Judicone, Sophie Laroumagne, Stéphane Robert, et al.. Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions. Oncotarget, Impact journals, 2016, 7, pp.3357-66. ⟨hal-01455550⟩

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