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Aminoacylase 1-catalysed deacetylation of bioactives epoxides mycotoxin-derived mercapturates; 3,4-epoxyprecocenes as models of cytotoxic epoxides

Pierre Stocker 1 Jean Michel Brunel 2 Leandro Rezende 3 Antonia Tavares-Do Amaral 3 Xavier Morelli 4 Phillipe Roche 4 Nicolas Vidal 1 Thierry Giardina 1 Josette Perrier 1
1 ISM2-AD2M - CNRS UMR 6263 - Institut des Sciences Moléculaires de Marseille
2 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
3 USP - Universidade de São Paulo
4 IMR - Interactions et Modulateurs de Réponses
Abstract : The mycotoxin aflatoxin B1 (AFB1) is a carcinogenic food contaminant which is metabolically activated by epoxydation. The metabolism of mycotoxins via the mercapturate metabolic pathway was shown, in general, to lead to their detoxication. Mercapturic acids thus formed (S-substitued-N-acetyl-L-cysteines) may be accumulated in the kidney and either excreted in the urine or desacetylated by Acylase 1 (ACY1) to yield cysteine S-conjugates. To be toxic, the N-acetyl-L-cysteine-S-conjugates first have to undergo deacetylation by ACY 1. The specificity and rate of mercapturic acid deacetylation may determine the toxicity, however the exact deacetylation processes involved are not well known. The aim of this study was to investigate the role of ACY1 in the toxicity of some bioactive epoxides from Aflatoxin B1. We characterized the kinetic parameters of porcine kidney and human recombinant aminoacylase-1 towards some aromatic and aliphatic-derived mercapturates analogue of mycotoxin mercapturic acids and 3,4-epoxyprecocene, a bioactive epoxide derivated from aflatoxin. The deacetylation of mercapturated substrates was followed both by reverse phase HPLC and by TNBS method. Catalytic activity was discussed in a structure function relationship. Ours results indicate for the first time that aminoacylase-1 could play an important role in deacetylating mercapturate metabolites of aflatoxin analogues and this process may be in relation with their cyto- and nephrotoxicity in human. (C) 2012 Published by Elsevier Masson SAS.
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https://hal-amu.archives-ouvertes.fr/hal-01460427
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Submitted on : Tuesday, February 7, 2017 - 4:40:00 PM
Last modification on : Thursday, December 16, 2021 - 3:56:40 AM

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UNIV-AMU | CNRS | ISM2 | SORBONNE-UNIVERSITE | INSERM | INC-CNRS

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Pierre Stocker, Jean Michel Brunel, Leandro Rezende, Antonia Tavares-Do Amaral, Xavier Morelli, et al.. Aminoacylase 1-catalysed deacetylation of bioactives epoxides mycotoxin-derived mercapturates; 3,4-epoxyprecocenes as models of cytotoxic epoxides. Biochimie, Elsevier, 2012, 94 (8), pp.1668-1675. ⟨10.1016/j.biochi.2012.01.006⟩. ⟨hal-01460427⟩

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