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Successful MALDI mass spectrometry of poly(4-vinylpyridine) using a solvent-free sample preparation

Abstract : The position of nitrogen atoms in poly(4-vinylpyridine) (P4VP), compared to their poly(2-vinylpyridine) (P2VP) isomers, explains most difficulties faced when determining their molecular weight parameters by size exclusion chromatography, due to oligomers strongly adsorbing onto stationary phases. The high accessibility of these nitrogen atoms is shown here to be challenging also in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), probably accounting for the lack of mass data reported for P4VP in the literature. Using a “bottom-up” strategy starting from small oligomers prior to consider high molecular weight polymers, P4VP species were observed here to strongly interact with acidic matrix molecules such as 2,5-DHB when preparing MALDI sample using the traditional dried-droplet procedure. In contrast, MALDI best performed when using a solvent-free sample preparation involving a non-acidic matrix such as DCTB. Accordingly, reliable MS determination of molecular weight parameters could be achieved for P4VP up to about 40 kDa, as validated by diffusion NMR experiments.
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https://hal-amu.archives-ouvertes.fr/hal-01460556
Contributor : Didier Gigmes <>
Submitted on : Tuesday, February 7, 2017 - 5:01:33 PM
Last modification on : Thursday, July 9, 2020 - 1:12:02 PM

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Christophe Chendo, Marion Rollet, Trang N. T. Phan, Stéphane Viel, Didier Gigmes, et al.. Successful MALDI mass spectrometry of poly(4-vinylpyridine) using a solvent-free sample preparation. International Journal of Mass Spectrometry, Elsevier, 2015, 376, pp.90--96. ⟨10.1016/j.ijms.2014.12.004⟩. ⟨hal-01460556⟩

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