Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon - Archive ouverte HAL Access content directly
Journal Articles Transfusion Year : 2015

Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon

Abstract

BACKGROUNDA new Marseilleviridae virus family member, giant blood Marseille-like (GBM) virus, was recently reported in persons from France in the serum of an infant with adenitis, in the blood of 4% of healthy blood donors, and in 9% of multiply transfused thalassemia patients. These results suggested the presence of a nucleocytoplasmic large DNA virus potentially transmissible by blood product transfusion. STUDY DESIGN AND METHODSTo investigate this possibility we tested the plasma from 113 US blood donors and 74 multiply transfused Cameroon patients for GBM viral DNA using highly sensitive polymerase chain reaction (PCR) assays. RESULTSGBM DNA was not detected by nested PCR in any of these 187 human specimens. CONCLUSIONSFurther testing is required to confirm the occurrence of human GBM virus infections.

Dates and versions

hal-01460644 , version 1 (07-02-2017)

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Tung Gia Phan, Christelle Desnues, William M. Switzer, Cyrille F. Djoko, Bradley S. Schneider, et al.. Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon. Transfusion, 2015, 55 (6), pp.1256--1262. ⟨10.1111/trf.12997⟩. ⟨hal-01460644⟩
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