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Evaluation of a new extraction protocol for yeast identification by mass spectrometry

Abstract : In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.
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Submitted on : Thursday, September 30, 2021 - 9:12:18 PM
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Frédérique Gouriet, Farida Ghiab, Carine Couderc, Fadi Bittar, Hervé Tissot Dupont, et al.. Evaluation of a new extraction protocol for yeast identification by mass spectrometry. Journal of Microbiological Methods, Elsevier, 2016, 129, pp.61-65. ⟨10.1016/j.mimet.2016.08.001⟩. ⟨hal-01478536⟩



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