Evaluation of a new extraction protocol for yeast identification by mass spectrometry

Frédérique Gouriet 1 Farida Ghiab Carine Couderc 1 Fadi Bittar 1 Hervé Tissot Dupont Christophe Flaudrops 1 Jean-Paul Casalta 1 Bissoume Sambe-Ba Bécaye Fall 2 Didier Raoult 3, 1 Florence Fenollar 1
1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2 LAMIH - Laboratoire d'Automatique, de Mécanique et d'Informatique industrielles et Humaines - UMR 8201
3 IHU AMU - Institut Hospitalier Universitaire Méditerranée Infection
Abstract : In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.
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Submitted on : Tuesday, February 28, 2017 - 11:06:54 AM
Last modification on : Friday, December 7, 2018 - 12:50:03 PM

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UNIV-AMU | PHARMA-AMU | UNIV-VALENCIENNES

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Frédérique Gouriet, Farida Ghiab, Carine Couderc, Fadi Bittar, Hervé Tissot Dupont, et al.. Evaluation of a new extraction protocol for yeast identification by mass spectrometry. Journal of Microbiological Methods, Elsevier, 2016, 129, pp.61 - 65. ⟨10.1016/j.mimet.2016.08.001⟩. ⟨hal-01478536⟩

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