Skip to Main content Skip to Navigation
Journal articles

Regulation of end-binding protein EB1 in the control of microtubule dynamics

Anne Nehlig Angie Molina Sylvie Rodrigues-Ferreira Stéphane Honoré 1, 2, 3 Clara Nahmias
1 CRO2 - Centre de Recherches en Oncologie biologique et Oncopharmacologie
2 TIMONE - Hôpital de la Timone [CHU - APHM]
3 INSERM - Institut National de la Santé et de la Recherche Médicale
Abstract : The regulation of microtubule dynamics is critical to ensure essential cell functions, such as proper segregation of chromosomes during mitosis or cell polarity and migration. End-binding protein 1 (EB1) is a plus-end-tracking protein (+TIP) that accumulates at growing microtubule ends and plays a pivotal role in the regulation of microtubule dynamics. EB1 autonomously binds an extended tubulin-GTP/GDP-Pi structure at growing microtubule ends and acts as a molecular scaffold that recruits a large number of regulatory +TIPs through interaction with CAP-Gly or SxIP motifs. While extensive studies have focused on the structure of EB1-interacting site at microtubule ends and its role as a molecular platform, the mechanisms involved in the negative regulation of EB1 have only started to emerge and remain poorly understood. In this review, we summarize recent studies showing that EB1 association with MT ends is regulated by post-translational modifications and affected by microtubule-targeting agents. We also present recent findings that structural MAPs, that have no tip-tracking activity, physically interact with EB1 to prevent its accumulation at microtubule plus ends. These observations point out a novel concept of "endogenous EB1 antagonists" and emphasize the importance of finely regulating EB1 function at growing microtubule ends.
Keywords : EB3 EB1 +TIP MAPs Phosphorylation Microtubule-targeting agents
Document type :
Journal articles
Complete list of metadata
https://hal-amu.archives-ouvertes.fr/hal-01478919
Contributor : Stéphane Honore Connect in order to contact the contributor
Submitted on : Tuesday, February 28, 2017 - 2:46:42 PM
Last modification on : Tuesday, October 19, 2021 - 10:59:04 PM

Identifiers

Collections

UNIV-AMU

Citation

Anne Nehlig, Angie Molina, Sylvie Rodrigues-Ferreira, Stéphane Honoré, Clara Nahmias. Regulation of end-binding protein EB1 in the control of microtubule dynamics. Cellular and Molecular Life Sciences, Springer Verlag, 2017, 74 (13), pp.2381-2393. ⟨10.1007/s00018-017-2476-2⟩. ⟨hal-01478919⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

115