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The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression

Abstract : The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes—Crip, Crp2, and Mta1—are located ≈70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′ Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
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Submitted on : Thursday, December 6, 2018 - 10:06:52 AM
Last modification on : Saturday, March 5, 2022 - 3:18:00 AM
Long-term archiving on: : Thursday, March 7, 2019 - 1:25:16 PM

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Jie Zhou, Nasrin Ashouian, Marc Délépine, Fumihiko Matsuda, Christophe Chevillard, et al.. The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2002, 99 (21), pp.13693-13698. ⟨10.1073/pnas.212392399⟩. ⟨hal-01592717⟩

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