High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2015

High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq

Résumé

Cell-type specific regulation of gene expression requires the activation of promoters by distal genomic elements defined as enhancers. The identification and the characterization of enhancers are challenging in mammals due to their genome complexity. Here we develop CapStarr-Seq, a novel high-throughput strategy to quantitatively assess enhancer activity in mammals. This approach couples capture of regions of interest to previously developed Starr-seq technique. Extensive assessment of CapStarr-seq demonstrates accurate quantification of enhancer activity. Furthermore, we find that enhancer strength is associated with binding complexity of tissue-specific transcription factors and super-enhancers, while additive enhancer activity isolates key genes involved in cell identity and function. The CapStarr-Seq thus provides a fast and cost-effective approach to assess the activity of potential enhancers for a given cell type and will be helpful in decrypting transcription regulation mechanisms.
Fichier principal
Vignette du fichier
ncomms7905.pdf (2.17 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01614948 , version 1 (09-01-2020)

Identifiants

Citer

Laurent Vanhille, Aurélien Griffon, Muhammad Ahmad Maqbool, Joaquin Zacarias-Cabeza, Lan T.M. Dao, et al.. High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq. Nature Communications, 2015, 6, pp.6905. ⟨10.1038/ncomms7905⟩. ⟨hal-01614948⟩
255 Consultations
103 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More