Skip to Main content Skip to Navigation
Journal articles

Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD

Abstract : A new aporphine glycoside (1), named " angkorwatine " , and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-β-D-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8) and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r 2 >0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39−4.18 µg/mL, 2.01−30.72 µg/mL and 4.29−64.42 µg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.
Complete list of metadatas

Cited literature [16 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01765806
Contributor : Stéphane Greff <>
Submitted on : Thursday, April 19, 2018 - 4:49:32 PM
Last modification on : Saturday, October 3, 2020 - 3:11:56 AM
Long-term archiving on: : Tuesday, September 18, 2018 - 3:10:42 PM

File

Dary_et_al_2017.pdf
Files produced by the author(s)

Identifiers

Citation

Chhavarath Dary, Sok-Siya Bun, Gaëtan Herbette, Fathi Mabrouki, Hot Bun, et al.. Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD. Natural Product Research, Taylor & Francis, 2016, 31 (7), pp.802-809. ⟨10.1080/14786419.2016.1247077⟩. ⟨hal-01765806⟩

Share

Metrics

Record views

275

Files downloads

486