Skip to Main content Skip to Navigation
New interface
Book sections

Large complexes: Cloning strategy, production and purification

Abstract : Membrane proteins can assemble and form complexes in the cell envelope. In Gram-negative bacteria, a number of multiprotein complexes including secretion systems, efflux pumps, molecular motors or pilus assembly machines comprise proteins from the inner and the outer membrane. Beside the structures of isolated soluble domains, only few atomic structures of these assembled molecular machines have been elucidated. To better understand the function and to solve the structure of protein complexes, it is thus necessary to design dedicated production and purification processes. Here, we present cloning procedures to overproduce membrane proteins into E. coli cells and describe the cloning and purification strategy for the Type VI secretion TssJLM membrane complex.
Document type :
Book sections
Complete list of metadata

Cited literature [30 references]  Display  Hide  Download
Contributor : roland lloubes Connect in order to contact the contributor
Submitted on : Wednesday, May 9, 2018 - 11:02:42 AM
Last modification on : Wednesday, October 27, 2021 - 10:00:07 AM
Long-term archiving on: : Tuesday, September 25, 2018 - 4:21:48 AM


Files produced by the author(s)




Eric Durand, Roland Lloubès. Large complexes: Cloning strategy, production and purification. Methods in Molecular Biology: Bacterial protein secretion systems, 2017, ⟨10.1007/978-1-4939-7033-9_24⟩. ⟨hal-01788569⟩



Record views


Files downloads