Skip to Main content Skip to Navigation
Journal articles

Functional and physical interaction between yeast Hsp90 and Hsp70

Abstract : Heat shock protein 90 (Hsp90) is a highly conserved ATP-dependent molecular chaperone that is essential in eukaryotes. It is required for the activation and stabilization of more than 200 client proteins, including many kinases and steroid hormone receptors involved in cell-signaling pathways. Hsp90 chaperone activity requires collaboration with a subset of the many Hsp90 cochaperones, including the Hsp70 chaperone. In higher eukaryotes, the collaboration between Hsp90 and Hsp70 is indirect and involves Hop, a cochaperone that interacts with both Hsp90 and Hsp70. Here we show that yeast Hsp90 (Hsp82) and yeast Hsp70 (Ssa1), directly interact in vitro in the absence of the yeast Hop homolog (Sti1), and identify a region in the middle domain of yeast Hsp90 that is required for the interaction. In vivo results using Hsp90 substitution mutants showed that several residues in this region were important or essential for growth at high temperature. Moreover, mutants in this region were defective in interaction with Hsp70 in cell lysates. In vitro, the purified Hsp82 mutant proteins were defective in direct physical interaction with Ssa1 and in protein remodeling in collaboration with Ssa1 and cochaperones. This region of Hsp90 is also important for interactions with several Hsp90 cochaperones and client proteins , suggesting that collaboration between Hsp70 and Hsp90 in protein remodeling may be modulated through competition between Hsp70 and Hsp90 cochaperones for the interaction surface. Ssa1 | Hsp82 | molecular chaperones | Sti1 | Ydj1.
Complete list of metadatas

Cited literature [64 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01890402
Contributor : Laure Azzopardi <>
Submitted on : Monday, October 8, 2018 - 3:48:41 PM
Last modification on : Wednesday, December 4, 2019 - 11:14:05 AM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Andrea Kravats, Joel Hoskins, Michael Reidy, Jill Johnson, Shannon Doyle, et al.. Functional and physical interaction between yeast Hsp90 and Hsp70. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2018, 115 (10), pp.E2210 - E2219. ⟨10.1073/pnas.1719969115⟩. ⟨hal-01890402⟩

Share

Metrics

Record views

83

Files downloads

84