Comparative evaluation of the UMIC Colistine kit to assess MIC of colistin of gram-negative rods

Lucie Bardet (1) , Liliane Okdah (2) , Stéphanie Le Page (1) , Sophie Alexandra Baron (1) , Jean-Marc Rolain (3)

1 AMU PHARM - Aix-Marseille Université - Faculté de pharmacie
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
3 MEPHI - Microbes évolution phylogénie et infections

Background The recent description of the first plasmid-mediated colistin-resistant gene mcr-1, conferring transferable and low-level resistance to colistin, raised concern about the need to implement a rapid and reliable screening method to detect colistin-resistant clinical isolates. The only valid method to assess the MIC of colistin is the broth microdilution according to the joint CLSI-EUCAST Polymyxin Breakpoints Working Group. UMIC Colistine is a ready-to-use broth microdilution kit developed to easily assess colistin MIC by proposing unitary polystyrene strips containing 11 concentrations of dehydrated colistin. Here, we evaluated the UMIC Colistine kit on 235 Gram-negative rods (176 Enterobacterales, including 70 harboring a mcr gene, and 59 non-fermentative), through comparison to the reference broth microdilution method prepared in accordance with EN ISO 20776-1:2006 standard. Reproducibility of the UMIC Colistine was assayed with the three recommended quality control strains E. coli ATCC 25922, E. coli NCTC 13846 (mcr-1 positive), and P. aeruginosa ATCC 27853, as for stability testing. Results Categorical agreement was 100% with 63.4% (n = 149) of colistin-resistant strains, and 36.6% (n = 86) of colistin-susceptible strains with both methods (S ≤ 2 μg/mL and R > 2 μg/mL). No major error or very major error was reported. Essential agreement was 94.0% (n = 221), and 100% for detection of colistin-resistant strains as compared to the reference method. Pearson’s correlation between UMIC Colistine and the reference method was 0.98. Reproducibility of the UMIC Colistine system was 97.8% with MICs of the quality control strains within the target ranges. However, some isolates had lower MIC with UMIC Colistine, but that did not change their categorization as colistin-susceptible, and this phenomenon should be further explored. Conclusions The UMIC Colistine kit is an easy to perform unitary device that showed excellent results when compared to the reference method. The UMIC Colistine system is a rapid and reliable broth microdilution method that is suitable to assess the colistin MIC of clinical isolates in clinical microbiology laboratories.

Mots clés: Colistin; Susceptibility testing; Polymyxin; UMIC; Enterobacteriaceae; Mcr-1; Broth microdilution; Detection method; Resistance; MIC

Domaines: Bactériologie; Maladies émergentes; Maladies infectieuses; Médicaments

Lucie Bardet, Liliane Okdah, Stéphanie Le Page, Sophie Alexandra Baron, Jean-Marc Rolain. Comparative evaluation of the UMIC Colistine kit to assess MIC of colistin of gram-negative rods. BMC Microbiology, 2019, 19 (1), ⟨10.1186/s12866-019-1424-8⟩. ⟨hal-02202232⟩