Skip to Main content Skip to Navigation
Journal articles

Decrypting the environmental sources of Mycobacterium canettii by high-throughput biochemical profiling

Abstract : Mycobacterium canettii is a smooth bacillus related to the Mycobacterium tuberculosis complex. It causes lymph nodes and pulmonary tuberculosis in patients living in countries of the Horn of Africa, including Djibouti. The environmental reservoirs of M. canettii are still unknown. We aimed to further decrypt these potential reservoirs by using an original approach of High-Throughput Carbon and Azote Substrate Profiling. The Biolog Phenotype profiling was performed on six clinical strains of M. canettii and one M. tuberculosis strain was used as a positive control. The experiments were duplicated and authenticated by negative controls. While M. tuberculosis metabolized 22/190 (11%) carbon substrates and 3/95 (3%) nitrogen substrates, 17/190 (8.9%) carbon substrates and three nitrogen substrates were metabolized by the six M. canettii strains forming the so-called corebiologome. A total at 16 carbon substrates and three nitrogen substrates were metabolized in common by M. tuberculosis and the six M. canettii strains. Moreover, at least one M. canettii strain metabo-lized 36/190 (19%) carbon substrates and 3/95 (3%) nitrogen substrates for a total of 39/285 (13%) substrates. Classifying these carbon and nitrogen substrates into ten potential environmental sources (plants, fruits and vegetables, bacteria, algae, fungi, nematodes, mol-lusks, mammals, insects and inanimate environment) significantly associated carbon and nitrogen substrates metabolized by at least one M. canettii strain with plants (p = 0.006). These results suggest that some plants endemic in the Horn of Africa may serve as ecological niches for M. canettii. Further ethnobotanical studies will indicate plant usages by local populations, then guiding field microbiological investigations in order to prove the definite environmental reservoirs of this opportunistic tuberculous pathogen.
Document type :
Journal articles
Complete list of metadatas

Cited literature [44 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-02279611
Contributor : Administrateur Hal Amu <>
Submitted on : Thursday, September 5, 2019 - 2:15:55 PM
Last modification on : Friday, June 12, 2020 - 3:40:08 PM
Long-term archiving on: : Thursday, February 6, 2020 - 11:01:05 AM

File

journal.pone.0222078.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Ahmed Loukil, Feriel Bouzid, Djaltou Aboubaker Osman, Michel Drancourt. Decrypting the environmental sources of Mycobacterium canettii by high-throughput biochemical profiling. PLoS ONE, Public Library of Science, 2019, 14 (9), pp.e0222078. ⟨10.1371/journal.pone.0222078⟩. ⟨hal-02279611⟩

Share

Metrics

Record views

78

Files downloads

168