TRAIL/Apo2L Mediates the Release of Procoagulant Endothelial Microparticles Induced by Thrombin In Vitro A Potential Mechanism Linking Inflammation and Coagulation

Stephanie Simoncini 1 Makon-Sébastien Njock 1 Stéphane Robert 1 Laurence Camoin-Jau 1, 2 José Sampol 1 Jean Robert Harlé 2 Catherine N'Guyen 3 Francoise Dignat-George 1, 2 Francine Anfosso 1
1 Physiopathologie de l'Endothelium
VRCM - Vascular research center of Marseille
2 LA CONCEPTION - Hôpital de la Conception [CHU - APHM]
3 TAGC - Theories and Approaches of Genomic Complexity
Abstract : Microparticles are small vesicles playing a crucial role in cell communication by promoting prothrombotic and proinflammatory responses. However, the molecular mechanisms underlying their release are still elusive. We previously established that thrombin promoted the generation of endothelial microparticles (EMPs). In the present study, gene profiling identified TRAIL/Apo2L, a cytokine belonging to the tumor necrosis factor-␣ superfamily, as a target of thrombin. Thrombin increased the expression of cell-associated and soluble forms of TRAIL (sTRAIL) in HMEC-1 cells and human umbilical vein endothelial cells (HUVECs). Blocking TRAIL by specific antibodies or by small interfering RNA reduced both the number and the procoagulant activity of EMPs released by thrombin. Consistent with an involvement of sTRAIL in thrombin-induced EMP release, we showed that (1) exogenously added sTRAIL generated procoagulant EMPs; (2) supernatants from thrombin-stimulated endothelial cells induced EMP release by HMEC-1 cells and HUVECs, whereas those recovered from TRAIL knockdown endothelial cells displayed no effect. TRAIL/ TRAIL-R2 complex mediated EMP release by initiating the recruitment of adaptor proteins and the activation of nuclear factor B. Moreover, sTRAIL modulated intercellular adhesion molecule-1 and interleukin-8 expression induced by thrombin by a downstream pathway involving nuclear factor B activation. Our data reveal a novel mechanism controlling EMP release and identify TRAIL as a key partner in the pathway linking coagulation and inflammation elicited by thrombin. (Circ Res. 2009;104:943-951.)
Keywords : coagulation inflammation cell signaling vesiculation endothelium thrombosis
Document type :
Journal articles
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download
https://hal-amu.archives-ouvertes.fr/hal-02478114
Contributor : Administrateur Hal Amu <>
Submitted on : Thursday, February 13, 2020 - 5:14:11 PM
Last modification on : Friday, February 14, 2020 - 1:35:53 AM

File

TRAIL_Apo2L.pdf
Files produced by the author(s)

Identifiers

Collections

UNIV-AMU

Citation

Stephanie Simoncini, Makon-Sébastien Njock, Stéphane Robert, Laurence Camoin-Jau, José Sampol, et al.. TRAIL/Apo2L Mediates the Release of Procoagulant Endothelial Microparticles Induced by Thrombin In Vitro A Potential Mechanism Linking Inflammation and Coagulation. Circulation Research, American Heart Association, 2009, 104 (8), pp.943-951. ⟨10.1161/CIRCRESAHA.108.183285⟩. ⟨hal-02478114⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

43

Files downloads

9

Contact

Informations

CCSD