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Recombinant HCV NS3 and NS5B enzymes exhibit multiple posttranslational modifications for potential regulation

Abstract : Posttranslational modification (PTM) of proteins is critical to modulate protein function and to improve the functional diversity of polypeptides. In this report, we have analyzed the PTM of both hepatitis C virus NS3 and NS5B enzyme proteins, upon their individual expression in insect cells under the baculovirus expression system. Using mass spectrometry, we present evidence that these recombinant proteins exhibit diverse covalent modifications on certain amino acid side chains, such as phosphorylation, ubiquitination, and acetylation. Although the functional implications of these PTM must be further addressed, these data may prove useful toward the understanding of the complex regulation of these key viral enzymes and to uncover novel potential targets for antiviral design.
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https://hal-amu.archives-ouvertes.fr/hal-02613462
Contributor : Julien Caugant <>
Submitted on : Wednesday, May 20, 2020 - 10:39:58 AM
Last modification on : Thursday, May 21, 2020 - 1:39:53 AM

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Sergio Hernández, Ariel Díaz, Alejandra Loyola, Rodrigo Villanueva. Recombinant HCV NS3 and NS5B enzymes exhibit multiple posttranslational modifications for potential regulation. Virus Genes, Springer Verlag, 2019, 55 (2), pp.227-232. ⟨10.1007/s11262-019-01638-2⟩. ⟨hal-02613462⟩

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